Bioinformatics institute

IBRI Speak to an expert: +91 9999509892
IBRI email: info@ibri.org.in

Indian Biosciences and Research Institute

High Pressure Liquid Cromatography

Module I: Introduction to HPLC

• Basic principles of HPLC
• Introduction to HPLC Pump
• Introduction to Auto Samplers and Injectors
• Introduction to HPLC Detectors
• Introduction to Chromatography Software

Module II: HPLC Troubleshooting & Maintenance

• Identification of Issues
• Maintenance of Pumps , Autosamplers & Detectors
• Calibration of Pump Flow , Autosampler Volume and Detector Wavelength
• Optimization of Chromatograph – Base Line , Peak Shape , Retention Time Drift etc

Module III: HPLC Method Development

• Defining the resolution, run time, specificity, accuracy, precision, limit of detection, etc
• Identification of key variables for retention, selectivity, and efficiency
• Column selection & handling
• Sample concentration, injection solvent, injection solvents
• Reverse phase HPLC
• HPLC Solvent Selection
• Ion Pair Chromatography
• Gradient HPLC
• Validation of HPLC Methods
• HPLC Solvent Selection

Module IV: Separation of Biopharmaceuticals through HPLC

• Column and mobile phase selection for reversed-phase, ion-exchange and size-exclusion chromatography
• Sample Preparation
• Column selection
• Run HPLC
• Peak Identification
• Analysis

Duration:

30 Days

Technical Assistance
& Laboratory Cost

Rs 6,000/-
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